Hatchery personnel found that 72% of the triploid eggs survived from the time the eggs were taken until the fish started to feed whereas only 54% of the diploid eggs survived until the fish started feeding. In 2013, the Story Hatchery conducted its own experiment to compare survivability of diploid versus triploid tiger trout eggs. Hatchery personnel are unable to explain why inducing triploidy in tiger trout eggs creates more viable and successful eggs, but studies have proven that it does. The triploid process actually produces more viable tiger trout eggs than if we left them as diploid eggs. By applying pressure during that exact time, the hatchery is able to produce triploid eggs. Previous studies have found that it is during this five minute period at 40 minutes post fertilization the third chromosome is kicked out of the egg. At exactly 40 minutes post fertilization, the group of eggs are placed into the chamber and are subjected to 9,500 psi of pressure for five minutes. After the eggs from the female and milt from the male are mixed to fertilize the eggs, the group of eggs is left to develop for 40 minutes. The Story Hatchery uses a small pressure chamber to induce triploidy in the eggs. Using pressure, at precisely the right time in the egg development, the chromosome is retained resulting in a sterile fish with three chromosomes.
To prevent the egg from rejecting the chromosome, Story Hatchery personnel use a pressure treatment method to retain the chromosome that would normally be kicked out of the egg during an early stage of egg development. Triploid tiger trout are created by forcing the egg to retain a chromosome that is normally ejected during egg development. Triploid fish simply have an extra copy of DNA that makes reproduction impossible. A triploid fish is not a genetically modified organism nor have the genes of the fish been manipulated or modified in any way. How do we know these fish are unable to reproduce? Well, the Story Hatchery creates what is called a triploid egg.Ī triploid fish has three sets of chromosomes, unlike a fertile fish that have two set of chromosomes (a diploid fish). One thing many people don’t know about tiger trout is that they are sterile and are unable to reproduce. The cross is completed with a female brown trout as attempts using a female brook trout by a male brown trout did not work very well and produced significantly lower quality eggs. For those unfamiliar with these hybrid fish, a tiger trout egg is produced by crossing a female brown trout with a male brook trout. The Story Hatchery began producing tiger trout eggs in the fall of 2011.
Recently at the Story Hatchery we've have been asked, how are these fish produced and where can I go to catch one of these feisty and colorful fish? Cheyenne - Tiger trout are a unique, relative newcomer to the Cowboy state.
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